Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 2 - SAMPLE PREPARATIONCarl Zeiss Introduction Lightsheet Z.14 000000-1790-528 02/2013Table 1Topic Subtopic Sample/ModelOrganismTechnique/ LSFMimplementationReferencePhysicsTechnical set up of MISERBStructured illuminationLight Sheet CharacteristicsImage formationImage View fusionFluorescent beadsMouse cochleaFluorescent beadsCaenorhabditis eleganslive sea urchin embryo, liveDanio rerio embryoMISERBsTSLIMSPIMDSLMLSFMFahrback et al, 2010Schroter et al, 2011Ritter et al, 2008Olarte et al, 2012Rubio-Guivernau et al,2012BiochemistryLaser MicrosurgeryMicrotubule dynamic instabilitymRNA nuclear exportHeterochromatin dynamicsImaging of engineered geneexpressionIn vitro microtubulesIn vitro microtubulesChironomus tentans SalivaryGlandsMCDK cells, DrosophilamelanogasterDrosophila melanogasterSPIMSPIMSPIMLSFM (FCS)SPIMEngelbrecht et al, 2007Keller et al, 2008Siebrasse et al, 2012Capoulade, 2011Ejsmont et al, 2009Microbiology Marine microbiology Various bacteria, protozoa etc. LSFM Fuchs et al, 2002Cell biologyAdaptive optics to improveimaging performanceIntracellular imagingNuclear protein localisationImaging large living samplesTumour spheroidsMammalian cell organellesCellular spheroidsMCDK cell cystswaoSPIMBessel beam planeilluminationSPIMSPIMJorand et al, 2012Planchon et al, 2011Zanacchi et al, 2011Verveer et al, 2007Plant BiologyLive imaging of root growthConsecutive imaging of verticallygrowing rootArabidopsis thalianaArabidopsis thalianaDSLMSPIMMaizel et al, 2011Sena et al, 2011DevelopmentalBiologyImaging of developing organsEmbryogenesis visualisationZebrafish developmentCell identity lineaging andneurodevelopmental imagingGene Expression: hour glassmodel verificationDanio rerio heart valveDrosophila embryoDanio rerioCaenorhabditis elegansDrosophila melanogasterSPIMSPIMmSPIMiSPIMSPIMScherz et al, 2008Huisken et al, 2004Kaufmann et al, 2012Wu et al, 2011Kalinka et al, 2010PhysiologyMiddle ear structure3D reconstruction of inner earBrain in vivo imaging3D reconstruction formorphological analysisScan of whole brainNeural network imagingSectioning of thick tissuesImaging neuronal activityImaging of immunolabelledreceptorsOptical sectioningPlastic Phantom StructureCavia porcellusMicrospheresBast's valveMouse brainMouse brainMouse cochlea/zebrafish innerear, brain/ rat brainMouse vomeronasal cellsMouseMeriones unguiculatuscochlea, Hippocampus reidihead, Xenopus laevis(HR) OPFOSOPFOS; LSFMminiSPIMOPFOSLSFMUltramicroscopeTSLIMOCPISPIMOPFOSBuytaert et al, 2007Hofman et al, 2009Engelbrecht et al, 2010Hofman et al, 2007Mertz and Kim, 2010Dodt et al, 2007Santi et al, 2009Holekamp et al, 2008Klohs et al, 2008Buytaert et al, 2012