Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 4 - SYSTEM OPERATIONCarl Zeiss Left Tool Area and Hardware Control Tools Lightsheet Z.188 000000-1790-528 02/20133.3.4 Processing – Image CalculatorLicensing Instrument standard optional  LSM  ELYRA  Lightsheet Z.1Tool accepts all types of image data recorded with the listed instruments. See fulldescription of tool for details.The Image calculator tool provides a calculator-style interface to apply arithmetic operators to theselected image.• To open the Image calculator tool, click on theImage calculator in the list of the Methodpanel in the Processing main tool tab.• Click on the triangle buttons to show or hidethe Method Parameters and Preview panels(Fig. 130) for the function.The two Select buttons in the Selection panelselect the active image in the Image Display asInput Image and Input Image 2. The OutputImage is automatically fed into a new imagedocument. A specific input channel from a multi-channel image or series can be selected using thepull down menus next to the Select buttons.The number in the First image display box, which can be edited, reduces the Input 2 Image series tothe selected first frames of a time series. This can be used to normalize a time series to the values of itsfirst image(s).After loading 2 input images (source images S1 and S2) specifying the input channels, the calculatorkeyboard in the Settings panel allows the definition of an operator or a combination of operators whichis represented by a formula in the formula display box. The operators will be inserted into the formula atthe cursor position. The formula can also be typed in directly. By pressing Input 1 or Input 2 sourceimage 1 or 2 will be loaded, respectively at the position of the cursor.Fig. 130 Processing – Image Calculator