Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 4 - SYSTEM OPERATIONCarl Zeiss Index Lightsheet Z.1254 000000-1790-528 02/2013New image document button ........................ 35OOffline calibration........................................ 101Online multiview processing ........................ 152Online ratio ................................................. 102Options ....................................................... 168OrthoDistance .................................................. 198Select ...................................................... 197Overlap coefficient ...................................... 229PPALM.......................................................... 113Convert to image ..................................... 123Import PALM molecules ........................... 122PALM ...................................................... 114Remove PALM outliers ............................. 121Particle tracking........................................... 124Pearson´s correlation coefficient ................... 228Pivot scan settings ......................................... 56Pre-Registration ........................................... 149Processing ..................................................... 82Adjust ..................................................... 159Average..................................................... 90Channel alignment .................................. 137Color-coded projection .............................. 87Copy ....................................................... 153Correlation .............................................. 105Filter .......................................................... 91HDR-imaging ........................................... 107ICS .......................................................... 155Image calculator ........................................ 88Ion concentration ...................................... 99Lightsheet processing............................... 142Linear unmixing ......................................... 94Maximum intensity projection .................... 86Modify series ........................................... 106PALM .......................................................113Particle tracking ........................................124SIM ..........................................................125Stitch .......................................................111Structured illumination .............................125Profile ..........................................................229PSF ..............................................................133QQuick setup ...................................................76RRaid elements ..............................................238Range indicator ..............................................58Ratiometric dyes ..........................................102Ratiometric dyes - Calibration .......................103Ratiometric dyes - Equation calibration .........104Ratiometric dyes - Online ratio ......................102Reference spectra...........................................98Registration ......................................... 147, 149Regularized inverse ......................................143Remove structure .........................................156Reuse ..........................................................186Right tool area ...............................................35SSave button ...................................................35Save status icon .............................................36Saving an image ..........................................249Scatter diagram ...........................................227Screen layout .................................................13Separate view controls ...................................34Series...........................................................216Setup manager ..............................................27Laser ..........................................................46Light path ..................................................47Shading correction .......................................162Shared view controls ......................................34Sharpen filter .................................................92

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