Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 4 - SYSTEM OPERATIONLightsheet Z.1 Index Carl Zeiss02/2013 000000-1790-528 253Initialization of positioning unit ........................ 9Intensity based alignment ............................ 149Interval time .................................................. 71Ion concentration .......................................... 99LLandmark alignment .................................... 148Large thumbnail view .................................... 34Laser blocking filter........................................ 50Laser control.................................................. 49Lasers off on exit ........................................... 12Left tool area ........................................... 27, 37Light Sheet Auto-Adjust ................................. 61Light Sheet illumination settings ..................... 56Light Sheet thickness ..................................... 54Lightsheet processing .................................. 142Blending .................................................. 152Deconvolution.......................................... 143Discrete fourier transform fusion .............. 142Dual side fusion ....................................... 142Fusion ...................................................... 152Input settings ........................................... 151Intensity based alignment ......................... 149Interpolation ............................................ 150Landmark alignment ................................ 148Mean fusion ............................................ 142Multiview processing ........................ 143, 147Online multiview processing ..................... 152Pre-Registration........................................ 149Registration ..................................... 147, 149Use registration from file .......................... 151Linear unmixing ............................................. 94Locate capillary .............................................. 38Locate sample ............................................... 38Locate tab ..................................................... 37Look Up Table (LUT)....................................... 58MMain tool tabs ............................................... 27Main toolbar ................................................. 23Maintain ............................................... 20, 163Administrator ............................................ 21Filters ...................................................... 167Objectives ................................................ 163Test grid .................................................... 21Manders coefficient ..................................... 229Manual detector alignment.......................... 175Map filter .................................................... 158Marker .................................................... 72, 74Master file ................................................... 236Maximum intensity projection ........................ 86Mean fusion ................................................ 142Mean of ROI................................................ 232Median filter ................................................. 91Menu bar ...................................................... 18Menu overview ............................................. 19Modify series ............................................... 106Moving average ............................................ 90Multichannel unmixing .................................. 98Multidimensional acquisition.................... 28, 44Information on experiment......................... 81Multiview .................................................. 76Time series................................................. 69Z-Stack ...................................................... 66Multidimensional image acquisition ............... 30Multiple columns layout ................................ 29MultiviewQuick setup ......................................... 76, 77Quick setup, rotations ................................ 81View.......................................................... 76Multiview processing ........................... 143, 147NNearest neighbour ....................................... 143